Allow to air dry.
Cleaning procedure for biological safety cabinet.
Biological safety cabinet sop bsc 01.
Cleaning a biosafety cabinet or bsc is an important function in terms of both containment and sterility.
5 1 3 once in a week remove adhered dust by wet mopping using soap solution.
Hepa stands for high efficiency particulate air.
The biological safety officer will determine the type of decontamination necessary.
μ microns in diameter with higher efficiency for both larger and.
This may take more or less time depending on the disinfectant and the microbiological agent used.
Aseptic technique and daily decontamination will eliminate the majority of contaminants.
Afterward wipe the surface with a clean dry cloth to remove the moisture.
10 easy steps to cleaning a contaminated surface in the biosafety cabinet.
If the biological safety cabinet is turned off between aseptic processes for routine maintenance or any other reason it should be operated long enough to allow complete purging of room air from the critical area for at least 30 minutes then cleaned and disinfected before use.
Typically surface decontamination is adequate however there are instances such as extensive repair inside the cabinet envelope or hepa filter replacement that will require gas decontamination.
In addition periodic and thorough decontamination routines including disinfecting all removable parts and surfaces is recommended by the manufacturer and is a good laboratory practice to reduce wear on the equipment and provide greater safety to you your samples and the environment.
It should be noted that the.
To begin apply disinfect to a towel and decontaminate and remove items from the area of the spill.
How to clean a biological safety cabinet lafw hood.
Spray the surfaces of all items placed into the cabinet with 70 ethanol to minimize the introduction of contaminants into the bsc.
Cleaning and disinfection 1.
How to clean a biological safety cabinet lafw hood.
The interior surfaces should also be wiped with an appropriate disinfectant that would kill any microorganisms that could be found in the cabinet.
Once the cabinet is empty you should allow 20 30 minutes to thoroughly decontaminate all interior work surfaces making sure to clean the back and side of the cabinet and the interior of the glass.
Put the waste from the spill into a biohazard bag inside the cabinet.
Once the cabinet has been operational for 15 minutes disinfect all interior surfaces of the cabinet thoroughly by spraying with 70 ethanol.
5 1 2 dedust the biosafety cabinet daily externally with a clean dry cloth.
Remember to wear appropriate ppe and leave the biosafety cabinet on during spill cleanup.
5 0 procedure 5 1 general cleaning procedure 5 1 1 ensure that the power supply of the biosafety cabinet is switched off.
